Carry Over Performance Verification

Calculate Carry Over (q%) for laboratory instruments

Test Entry Form

q is calculated from H2, L1 and L3. H1 and L2 are recorded as part of the a1, a2, b1, b2, b3 sequence.

Sequence Analysis Chart

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Results Summary

Sr. No.Test NameEquipmentDateH1H2L1L2L3q (%)Interpretation
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Interpretation & Reference

Carry over effect is calculated using the equation:

Carry Over = (L1 - L3) / (H2 - L3) × 100

"q" may also be called the 'carry-over ratio', and is sometimes expressed as a percentage value. It implies that the influence of carry-over effects on the analytical results is inversely proportional to the concentration difference of the component to be detected by the analytical procedure in the two specimens.

Carry-over within a particular procedure is of interest. It is dependent on the method, including all reagents applied. Therefore, carry-over effects, rather than carry-over itself, need to be determined. For this purpose, a sequence of specimens, usually with high (a) and low (b) concentrations of the analyte to be measured, are processed — for example a1, a2, b1, b2, b3.

Reference: R. Haeckel, Recommendations for definition and determination of carry over effects, Journal of Clinical Chemistry Vol. 10 No. 04 (Oct–Dec 1988, pp. 181–183)

Carry Over formula: q = (L1 - L3) / (H2 - L3) x 100. Acceptable if |q| < 1.0%
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